The Q-branch of the ν1+ν3+3ν4 cold band of 12C2H2 located at 8330 cm−1 has been observed under one kilometer absorption path length. The analysis leads to a line list for this isolated and strong Q-branch that is missing from spectroscopic databases. This work completes the large update of acetylene data in the ΔP = 13 spectral region (8200 and 8900 cm−1) made in spectroscopic databases (HITRAN and GEISA) in 2020 edition.